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KMID : 0545119930030040298
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Journal of Microbiology and Biotechnology
1993 Volume.3 No. 4 p.298 ~ p.302
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Pullulan Production from Starch Hydrolysate by Aureobasidium pullulans SH8646
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Shin, Yong Chul
Kim, Tae Un/Byun, Si Myung
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Abstract
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Pullulan was produced from starch hydrolysate with Aureobasidium pullulans SH8646. We could measure the correct amount of pullulan produced without the interference of starch from the culture supernatant by using a bacterial α-amylase treatment and ethanol: acetone (1:1) precipitation. When 5% acidhydrolyzed starch was used as a carbon source, the dry cell weights obtained were similar irrespective of DE values of starch hydrolysates. The dry cell weights of those on the starch hydrolysate media prepared with 0.1 N HCl treatment, were slightly higher (9.5∼10.5g/ℓ) than those on the starch hydrolysate media prepared with 1.0 N HCl (8.5∼9.5g/ℓ). And among the starch hydrolysates showing DE values lower than 50, maximum pullulan production of 15g/ℓ was obtained at DE 30∼40 starch hydrolysate but those showing DE values higher than 50, the pullulan production was increased with the increase of the DE value of starch hydrolysates. From the media containing 5%, 10%, and 15% starch hydrolysate (DE 25, 45, and 75), about 20∼34% pullulan yield was obtained and the maximum pullulan yield of 34% (17g/ℓ) was obtained from 5% DE 75 starch hydrolysate. The pullulan yields from starch hydrolysate media were much lower than those from glucose, maltose, maltotriose, and sucrose media.
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